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High Sensitivity 0.06mg/kg DBP ELISA Test Kit with High Recovery 75-105% for Rapid Detection in 30min

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High Sensitivity 0.06mg/kg DBP ELISA Test Kit with High Recovery 75-105% for Rapid Detection in 30min

High Sensitivity 0.06mg/kg DBP ELISA Test Kit with High Recovery 75-105% for Rapid Detection in 30min
High Sensitivity 0.06mg/kg DBP ELISA Test Kit with High Recovery 75-105% for Rapid Detection in 30min

Large Image :  High Sensitivity 0.06mg/kg DBP ELISA Test Kit with High Recovery 75-105% for Rapid Detection in 30min

Product Details:
Place of Origin: China
Brand Name: Chenhui
Model Number: CH580912
Payment & Shipping Terms:
Minimum Order Quantity: 1kits
Price: Negotiable
Packaging Details: color packing
Delivery Time: 5-8 work day
Payment Terms: T/T
Supply Ability: 1000 Kits

High Sensitivity 0.06mg/kg DBP ELISA Test Kit with High Recovery 75-105% for Rapid Detection in 30min

Description
Rapid: <30min High Recovery: 75-105%
High Sensitivity: 0.06mg/kg Detection Time: 30min
Storage Temperature: 2-8°C Specification: 96T
Accuracy: 100%±20%
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High Sensitivity 0.06mg/kg DBP ELISA Test Kit

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High Recovery 75-105% Dibutylphthalate Test Kit

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Rapid Detection 30min Food Safety ELISA Kit

High Sensitivity, High Recovery Rate Dibutylphthalate (DBP) ELISA Test Kit
This ELISA test kit is designed for the detection of Dibutylphthalate (DBP) in various edible oils including rapeseed oil, soybean oil, peanut oil, corn oil, and blended oil. The kit features single-point calibration capability for quantitative analysis and can test up to 47 samples simultaneously.
Product Code: CH580912
For data analysis when using single-point calibration, please contact us to request the dedicated Excel data analysis software.
Sample Pretreatment
Required Materials (Not Provided)
  • Dimethyl sulfoxide, analytical pure
  • Deionized water
  • 5mL glass centrifuge tube
  • 2mL glass vial
Pretreatment Procedure for Animal and Vegetable Edible Oils
  1. Weigh 1.5±0.01g of oil sample into a 5mL glass centrifuge tube, add 1.5mL of dimethyl sulfoxide and shake for 3 minutes
  2. Centrifuge at 4000rpm for 1 minute
  3. Discard the upper liquid layer, take 1mL of the lower layer and add to purification tube, then add 0.2mL of DBP purification reagent A and shake for 1 minute
  4. Centrifuge at 4000 RPM for 1 minute
  5. Take 0.1mL of the supernatant and add to 0.9mL of sample diluent, then vortex for 15 seconds
Sample dilution factor: 10
Operating Procedure
Before the experiment: Remove test kit from 2-8℃ refrigerator and balance at indoor temperature (20-28℃) for more than 1 hour!
Reagent Preparation
Washing solution (1×): Take 1 volume of 20× concentrated washing solution, add 19 volumes of deionized water or distilled water, mix well, and set aside. Prepare as needed.
Assay Procedure
  1. Remove appropriate number of balanced strips and return the rest to the aluminum film bag
  2. Add 150μL of DBP activation reagent to the microwells; tap the board frame gently
  3. Cover the plate, let stand at 20-28℃ for 5 minutes, then pour out and pat dry on plate cloth
  4. Add 50μL of standard/sample to corresponding microwells; add 50μL of enzyme conjugate and 50μL of antibody to all microwells; tap board frame gently
  5. Cover plate and incubate at 20-28℃ for 20 minutes. Pour out liquid from micropores
  6. Fill micropores with washing solution, soak for 15 seconds, then pour out. Repeat 4 times for total of 5 washes. Pat dry on racket cloth
  7. Add 100μL of substrate to microwells, gently tap plate rack, cover with plate tape, and incubate at 20-28℃ for 10 minutes
  8. Remove plate patch, add 100μL of stop solution to microwells, gently tap plate frame, and take reading at wavelength of 450/630nm
High Sensitivity 0.06mg/kg DBP ELISA Test Kit with High Recovery 75-105% for Rapid Detection in 30min 0

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G
G*a
Mexico Mar 4.2026
High accuracy, good stability, and simple and convenient operation.
Contact Details
Maoming Chenhui Biotechnology Co., Ltd.

Contact Person: Tina

Tel: +8615818799493

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