Single-point Standard Substance Aflatoxin B1 ELISA Test Kit

The unique features of the kit are:

• LOD:2μg/kg
• LOQ:2.5μg/kg .
• Quantitative Range:2.5-100μg/kg
• 20min
• recovery for certificated reference material:85%~110%

Reagent Preparation

• Extraction-solvent

The formulation of Extraction-solvent is as follow:

ethanol:distilled water=1:1(v/v); for exemple, ethanol 500mL+distilled water 500mL, then vortex homogeneous.

Cecreals, bran cereals, DDGS, corn gluten meal, corn germ, corn syrup and animal feed

A representative sample( according to accepted sampling techniques) should be ground and thoroughly mixed prior to proceeding with the extraction procedure.

• Weigh out 5g of the homogenize sample into a suitable vial and vortex thoroughly with 25mL of Extraction-solvent for 3min.
• Filter the extract with Whatman NO.1 filter(or equivalent), or centrifuge the extract at 4000×g for 3~5 minutes.
• An aliquot of 100μL the filtrate obtained after filtration(or 100μL the supernatant obtained after centifugation), is diluted with 300μL of the Universal Sample Diluent.
• Use 50μL of the diluted solution per well for the assay.

Note:

• For bran cereals extraction, it is recommended to extract the sample with 1:7(w/v) or more;
• the pH of the supernatant or the filtrate should be 6~8, can adjust it with 1N NaOH or 1N HCl
• The diluted solution can be used as the determination of deoxynivalenol, aflatoxin b1 and aflatoxin b1

Peanut meal

A representative sample( according to accepted sampling techniques) should be ground and thoroughly mixed prior to proceeding with the extraction procedure.

• Weigh out 5g of the homogenize sample into a suitable vial and vortex thoroughly with 10mL of acetonitrile for 3min.
• Filter the extract with Whatman NO.1 filter(or equivalent), or centrifuge the extract at 4000×g for 3~5 minutes.
• Evaporate clean the liquid of 0.2mL of the filtrate obtained after filtration(or 0.2mL the supernatant obtained after centifugation). Then resolve the remaining residue with 1mL of Extraction-solvent.
• An aliquot of 100μL the resolved solution, is diluted with 300μL of the Universal Sample Diluent.
• Use 50μL of the diluted solution per well for the assay.

Dilution factor:2