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Tetrodotoxin TTX ELISA Test Kit Easy To Operate And Takes A Short Time

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1.5ppb Stable Toxin Test Kits Tetrodotoxin TTX ELISA Test Kit

1.5ppb Stable Toxin Test Kits Tetrodotoxin TTX ELISA Test Kit

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Tetrodotoxin TTX ELISA Test Kit Easy To Operate And Takes A Short Time

Tetrodotoxin TTX ELISA Test Kit Easy To Operate And Takes A Short Time
Tetrodotoxin TTX ELISA Test Kit Easy To Operate And Takes A Short Time Tetrodotoxin TTX ELISA Test Kit Easy To Operate And Takes A Short Time Tetrodotoxin TTX ELISA Test Kit Easy To Operate And Takes A Short Time Tetrodotoxin TTX ELISA Test Kit Easy To Operate And Takes A Short Time Tetrodotoxin TTX ELISA Test Kit Easy To Operate And Takes A Short Time Tetrodotoxin TTX ELISA Test Kit Easy To Operate And Takes A Short Time

Large Image :  Tetrodotoxin TTX ELISA Test Kit Easy To Operate And Takes A Short Time

Product Details:
Place of Origin: China
Brand Name: Chenhui
Model Number: CH98016
Payment & Shipping Terms:
Minimum Order Quantity: 1kits
Price: Negotiable
Packaging Details: color packing
Delivery Time: 5-8 work day
Payment Terms: T/T
Supply Ability: 1000 Kits
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Tetrodotoxin TTX ELISA Test Kit Easy To Operate And Takes A Short Time

Description
Rapid: ＜30min High Recovery: 75-105%
High Sensitivity: 1.5ppb Detection Time: 1.5hours
Storage Temperature: 2-8°C Specification: 96T
Linear Interval: 1.5ng/mL~40.5ng/mL Sample Type: Food, Water, Soil

  Tetrodotoxin ELISA Kit

          CH98016

 

        Tetrodotoxin ELISA Kit provides a competitive enzyme immunoassay for the quantitative analysis of Tetrodotoxin in Pufferfish.Up to 96 tests or assaying about 42 samples in duplicate( assuming 12 wells for standards).

 

   

       

1.Sample Preparation

Reagent Preparation

  • Extraction-solvent

The formulation of Extraction-solvent is as follow: methanol:distilled water:glacial acetic acid=400:599:1; for exemple, methanol 40mL+distilled water 59.9mL +glacial acetic acid 0.1mL

  • 1×sample-diluent

Mix 1 volume of 4×sample-diluent with 3 volumes of distilled water.

 

Pufferfish(muscle, liver, skin)

  • Homogenize a reasonable amount of sample with a suitable mixer.
  • Weigh out 2g of the homogenize sample into a suitable vial and mix thoroughly with 10mL of Extraction-solvent.
  • Keep the vial staying in a 90℃ water bath for 10 minutes and during this procedure, shake the vial for 3 times .
  • Rinse the vial for 1minute with tap water to cool down the sample and then add 1mL of hexane, vortex for 30 seconds;
  • Centrifuge the sample at 4,000×g for 5 minutes, piptte 100μL of the interlayer liquid and add 300μL of 1×sample-diluent
  • Use 50μL of the sample solution per well for the assay.

Note: Dilution Factor :20

 
 
 

2.Assay Protocol

Preparation Before Testing

IMPORTANT:

  • All reagents should be brought up to room temperature before use (1 – 2 hours at 20 – 25°C / 68 – 77°F);
  • Solutions should be prepared just prior to ELISA test.
  • All reagents should be mixed by gently inverting or swirling prior to use.
  • Prepare volumes that are needed for the number of wells being run.
  • Do not return the reagents to the original stock tubes/bottles. Using disposable reservoirs when handling reagents can minimize the risk of contamination and is recommended.
  • Avoid running assays in direct sunlight or under or near air vents, as it may cause the changes of micro-environments between the wells.

 

Preparation of 1X Wash Solution

Mix 1 volume of 20X Wash Buffer concentrate with 19 volumes of distilled water.

Tag:

elisa test kits,

Toxin Test Kit,

toxins test

Contact Details
Maoming Chenhui Biotechnology Co., Ltd.

Contact Person: Tina

Tel: +8615818799493

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