Cylindrospermopsin ELISA Toxin Test Kits Elisa Test Kits 0.75ppb In Water Samples

Cylindrospermopsin ELISA Test Kit In Water Samples

summarize

This  M. coli toxin ELISA kit is suitable for the quantitative detection of M. coli toxin in water samples. Concentration is not required during sample processing. If necessary, positive samples can be verified by HPLC or other traditional methods.
 

Security specification
The substrate solution contains TMB and the termination solution contains dilute sulfuric acid. Avoid contact between skin and substrate solution and termination solution. Rinse with clean water in case of accidental contact.
Storage and stability
The ELISA test kit should be stored at 4-8 °C. Return to room temperature (20-25°C) before use. It can be used within the validity period.
 

principle

The  test kit works by directly competing the enzyme-linked immune response. Cylindrosporin is identified and detected by a specific antibody. When there is still a sample of columnetoxin and its analogues, then they will compete with columnetoxin-HRP and bind to the microcystin antibody in solution. The microcystin antibody binds to the sheep anti-mouse secondary antibody coated at the bottom of the microplate. A colorless substrate is added after a washing step, resulting in a color reaction. The color changes from blue to yellow after the reaction terminating solution is added. At 450nm, the concentration of microcystin in the sample was inversely proportional to the absorbed light intensity.

Limitations of the kit and possible cross-reactivity

A variety of organic and inorganic substances frequently present in water samples have been recently tested without interference with this kit. Interference caused by matrix reactions due to the compound's perishable nature is impossible to completely avoid. When the concentration of the following substances is not higher than 10,000 ppm, it has no effect on the test results of the kit: alumina, calcium chloride, calcium sulfate, manganese sulfate, magnesium sulfate, magnesium chloride, sodium chloride, phosphate, sodium thiosulfate. When the concentration of the following substances is not higher than 1,000 ppm, it has no effect on the detection results of the kit: sodium nitrate, zinc sulfate; Humic acid less than 100 ppm has no effect on the detection results. Copper chloride less than 10 ppm has no effect on the detection results. Improper operation may lead to incorrect test results, such as: incorrect kit storage conditions, wrong liquid addition sequence, the amount of reagents is not accurately added, the incubation time is too long or too short, the incubation temperature is too high or too low. The operation process is not easy to carry out in strong light. As with other testing methods, positive results are verified by other traditional methods.