Tetrodotoxin TTX ELISA Test Kit 1.5ppb High Sensitivity And Simple Operation

Reagent Preparation

• Extraction-solvent

The formulation of Extraction-solvent is as follow: methanol:distilled water:glacial acetic acid=400:599:1; for exemple, methanol 40mL+distilled water 59.9mL +glacial acetic acid 0.1mL

• 1×sample-diluent

Mix 1 volume of 4×sample-diluent with 3 volumes of distilled water.

Pufferfish(muscle, liver, skin)

• Homogenize a reasonable amount of sample with a suitable mixer.
• Weigh out 2g of the homogenize sample into a suitable vial and mix thoroughly with 10mL of Extraction-solvent.
• Keep the vial staying in a 90℃ water bath for 10 minutes and during this procedure, shake the vial for 3 times .
• Rinse the vial for 1minute with tap water to cool down the sample and then add 1mL of hexane, vortex for 30 seconds;
• Centrifuge the sample at 4,000×g for 5 minutes, piptte 100μL of the interlayer liquid and add 300μL of 1×sample-diluent
• Use 50μL of the sample solution per well for the assay.

Note: Dilution Factor :20