Saxitoxin PSP Mold Toxin Test ELISA Test Kit Water Samples Shellfish Samples

                                                                                            CH98013

       Saxitoxin (PSP) ELISA Test Kit Water Samples As Well As Shellfish Samples

Product Description

              Saxitoxin (PSP) ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of Saxitoxin in mussels and water samples. Saxitoxin, the principal component of paralytic shellfish poisons (PSP), blocks the sodium ion channels in nerve and muscle membranes.The lethal dose in humans is 1 to 3 mg. Numbness and respiratory paralysis can occur after oral ingestion of as little as 0.5 - 1.0mg saxitoxin. Saxitoxin residues can be determined by either GC-MS or HPLC. However, enzyme immunoassays (ELISA) show considerable advantages as it is faster and more sensitive.

           Saxitoxin (PSP) ELISA Test Kit enables international and government regulatory agencies, food manufacturers and processors, as well as quality assurance organizations, to detect saxitoxin in shellfish matrices and to satisfy customer concerns about food safety.

The unique features of the kit are:

§ High recovery (80%-105%), rapid (10 - 30 minutes), and cost-effective extraction methods

§ High sensitivity (0.1 ng/g or ppb) and low detection limit (100 ppb for mussel samples ).

§ High reproducibility.

§ A quick ELISA assay(less than 1.5 hours regardless of number of samples).

Procedure Overview

The test is a direct competitive ELISA based on the recognition of Okadaic Acid by specific antibodies. Okadaic Acid, when present in a sample, and an okadaic acid-enzyme-conjugate compete for the binding sites of rabbit anti-okadaic acid antibodies in solution. The okadaic acid antibodies are then bound by a second antibody (goat anti-rabbit) immobilized on the plate. After a washing step and addition of the substrate solution, a color signal is produced. The intensity of the blue color is inversely proportional to the concentration of Okadaic Acid present in the sample. The color reaction is stopped after a specified time and the color is evaluated using an ELISA reader. The concentrations of the samples are determined by interpolation using the standard curve constructed with each run.

Kit Contents, Storage and Shelf Life

           Saxitoxin (PSP) ELISA Test Kit has the capacity for 96 determinations or testing of 42 samples in duplicate (assuming 12 wells for standards). Return any unused microwells to the foil bag and reseal them with the desiccant provided in the original package. Store the kit at 2-8°C *. The shelf life is 12 months when the kit is properly stored.