Sarafloxacin ELISA Test Kit Used In Milk, Eggs, Feed, Fish And Shrimp, Honey, Meat, Offal, Serum And Urine

Product Description

           Sarafloxacin is a fluoroquinolone antibiotic which works by inhibition of bacterial DNA- topoisomerase II. It has been proposed for use in the drinking water of poultry to treat bacterial disease, and in fish feed to treat diseases such as furunculosis, vibriosis and enteric redmouth. The ELISA Test Kit enables government agencies, food manufacturers and quality assurance organizations to detect sarafloxacin in various sample types and to satisfy customer concerns about food safety.

The features of the kit are:

• Extraction of sarafloxacin from chicken, fish/shrimp and milk with a high recovery rate.
• A quick ELISA assay (50 minutes incubation time).
• High reproducibility.

Procedure Overview

The method is based on a competitive colorimetric ELISA assay. The drug of interest has been coated in the plate wells. During the analysis, sample is added along with the arafloxacin-horseradish peroxidase (Sarafloxacin-HRP) conjugate. If the sarafloxacin residue is present in the sample, it will compete for the sarafloxacin antibody, thereby preventing the Sarafloxacin-HRP from binding to the antibody. The resulting color intensity, after addition of substrate(TMB), has an inverse relationship with the target concentration in the sample.

Kit Contents, Storage and Shelf Life

             Sarafloxacin ELISA Test Kit has the capacity for 96 determinations or testing of 42 samples in duplicate (assuming 12 wells for standards). Return any unused microwells to the foil bag and reseal them with the desiccant provided in the original package. Store the kit at 2-8°C. The shelf life is 12 months when the kit is properly stored.