Cefoperazone ELISA Test Kit In Milk, Urine, Tissue (Liver, Kidney, And Muscle), Honey, Serum, Swipe Sample And Plasma Samples

Product Description

           Cefoperazone ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of Cefoperazone in milk, urine, tissue (liver, kidney, and muscle), honey, serum, swipe sample and plasma samples...

The features of the kit are:

• High recovery (>75%), low-cost rapid sample prep protocol (10 minutes - 1 hour)
• High sensitivity: 0.25 ng/gram = 0.25 ppb (parts per billion)
• Rapid assay protocol (less than 2 hours to process 40 samples in duplicate)
• High reproducibility (interplate and intraplate CV typically <10%)

Procedure Overview

The method is based on a competitive colorimetric ELISA assay. The drug of interest (Cefoperazone) has been coated in the plate wells. The sample to be tested is mixed with a high-affinity Cefoperazone capture protein and added to the well. If the Cefoperazone target is present in the sample, it will compete for the capture protein, thereby preventing the capture protein from binding to the drug attached to the well. The secondary antibody, tagged with a peroxidase enzyme, targets the capture protein bound to the drug coated on the plate wells. The resulting color intensity, after addition of substrate, has an inverse relationship with the target concentration in the sample. The levels of Cefoperazone in the sample are determined by comparison of the color intensity of unknown samples with the standard curve, which is made using the Cefoperazone standard provided in the kit.

Kit Contents, Storage and Shelf Life

        Cefoperazone ELISA Test Kit has the capacity for 96 determinations or testing of 42 samples in duplicate (assuming 12 wells for standards). Return any unused microwells to the foil bag and reseal them with the desiccant provided in the original package. Store the kit at 2-8°C. The shelf life is 12 months when the kit is properly stored.