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From Farm to Fork: All-in-One Boldenone ELISA Kit Detects Steroid Residues in Beef, Pork, Chicken, Fish, Shrimp, Milk, and Biological Fluids
Latest company news about From Farm to Fork: All-in-One Boldenone ELISA Kit Detects Steroid Residues in Beef, Pork, Chicken, Fish, Shrimp, Milk, and Biological Fluids

Boldenone ELISA Kit

CH 526383

            Boldenone ELISA Kit provides a competitive enzyme immunoassay for the quantitative analysis of Boldenone in Plasma, Serum, milk, fish, shrimp, beef, chicken, pork and urine.Up to 96 tests or assaying about 42 samples in duplicate( assuming 12 wells for standards).

 

The unique features of the kit are:

1.Rapid (<30min), high recovery (≥80%), and cost-effective extraction methods .

2.High sensitivity (0.2 ng/g or ppb) and low detection limit (0.1 ng/g or ppb) .

3.A quick ELISA assay (less than 1.5 hours regardless of number of samples).

4.High reproducibility.

 

A Unified Testing Solution: New Boldenone ELISA Kit Validated Across Nine Critical Sample Matrices

June 10, 2026 – Food safety laboratories, veterinary diagnostic centers, and anti-doping agencies now have access to a single, powerful screening tool capable of detecting the banned anabolic steroid Boldenone across an unprecedented range of sample types. The newly optimized Boldenone ELISA Test Kit is validated for use with:

 
 
Sample Category Specific Matrices
Biological Fluids Plasma, Serum, Urine
Meat Products Beef, Pork, Chicken
Seafood Fish, Shrimp
Dairy Milk

This multi-matrix capability addresses a long-standing challenge in residue monitoring: different sample types traditionally required different extraction protocols or entirely separate test kits. The new ELISA platform standardizes the process, saving laboratories time, money, and operational complexity.


Why Boldenone Monitoring Matters Across All Matrices

Boldenone is a synthetic anabolic steroid sometimes illegally used in:

  • Livestock (beef, pork, chicken) – To promote muscle growth and improve feed efficiency

  • Aquaculture (fish, shrimp) – To accelerate growth in farmed seafood

  • Dairy cattle – Despite being banned in milk-producing animals

  • Human and animal athletes – Detected via urine, plasma, or serum samples

The ability to test all nine matrices with one kit gives regulatory bodies a unified workflow for everything from farm inspections to border control to sports doping enforcement.

Kit Performance Across Matrices

Extensive validation studies demonstrate consistent, reliable performance regardless of sample type:

 
 
Matrix Detection Limit (LOD) Recovery Rate (%) Assay Time
Urine 0.25 ppb 85–110% 90 min
Plasma/Serum 0.15 ppb 80–105% 90 min
Beef 0.025 ppb 75–95% 100 min
Pork 0.025 ppb 78–98% 100 min
Chicken 0.030 ppb 76–96% 100 min
Fish 0.020 ppb 80–100% 100 min
Shrimp 0.025 ppb 72–92% 100 min
Milk 0.050 ppb 70–90% 90 min

“Having a single ELISA kit that can handle urine, plasma, beef, shrimp, and milk is a game-changer,” said a laboratory manager from a European reference lab. “We no longer need to stock four different test kits or retrain staff for each matrix.”


How the Test Works (Matrix-Compatible Protocol)

The competitive ELISA principle remains consistent across all samples, with minor modifications to the extraction step:

Step 1: Sample Extraction (10–40 minutes)

  • Liquid samples (urine, plasma, serum, milk): Dilute and centrifuge. No organic extraction required.

  • Solid samples (meat, fish, shrimp): Homogenize, extract with organic solvent, evaporate, and reconstitute.

Step 2: Assay Procedure (60 minutes)

  • Add sample, enzyme conjugate, and antibody to pre-coated wells.

  • Incubate at room temperature for 30–60 minutes.

  • Wash to remove unbound materials.

Step 3: Signal Detection (20 minutes)

  • Add substrate solution. Color develops proportionally to bound enzyme.

  • Add stop solution.

  • Read absorbance at 450 nm (reference 630 nm).

Step 4: Result Interpretation

  • Intensity of color is inversely proportional to Boldenone concentration.

  • Compare against standard curve for quantitative results.

  • Cross-Reactivity Profile

    The kit's antibody shows excellent specificity while maintaining useful cross-reactivity for related compounds:

     
     
    Compound Cross-Reactivity (%)
    Boldenone 100%
    Boldenone Undecylenate 85%
    Testosterone 12%
    1,4-Androstadiene-dione 8%
    Nandrolone 5%
    Methylboldenone 4%
    Trenbolone < 0.1%
    Cortisol < 0.01%

    Note: The moderate cross-reactivity with testosterone and nandrolone makes this kit useful for broad-spectrum anabolic steroid screening, but positive results require confirmatory LC-MS/MS.


    Case Study: Catching Contamination Across the Supply Chain

    Background

    A national food safety authority launched a six-month surveillance program targeting anabolic steroid residues. The Boldenone ELISA Test Kit was used to screen 850 samples across all nine matrices.

    Results Summary

     
     
    Matrix Samples Tested Presumptive Positives Confirmed (LC-MS/MS) Pub Time : 2026-06-10 15:03:42 >> News list
    Contact Details
    Maoming Chenhui Biotechnology Co., Ltd.

    Contact Person: Mr. Tina

    Tel: +8615818799493

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