Boldenone ELISA Kit
CH 526383
Boldenone ELISA Kit provides a competitive enzyme immunoassay for the quantitative analysis of Boldenone in Plasma, Serum, milk, fish, shrimp, beef, chicken, pork and urine.Up to 96 tests or assaying about 42 samples in duplicate( assuming 12 wells for standards).
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The unique features of the kit are: 1.Rapid (<30min), high recovery (≥80%), and cost-effective extraction methods . 2.High sensitivity (0.2 ng/g or ppb) and low detection limit (0.1 ng/g or ppb) . 3.A quick ELISA assay (less than 1.5 hours regardless of number of samples). 4.High reproducibility. |
June 10, 2026 – Food safety laboratories, veterinary diagnostic centers, and anti-doping agencies now have access to a single, powerful screening tool capable of detecting the banned anabolic steroid Boldenone across an unprecedented range of sample types. The newly optimized Boldenone ELISA Test Kit is validated for use with:
| Sample Category | Specific Matrices |
|---|---|
| Biological Fluids | Plasma, Serum, Urine |
| Meat Products | Beef, Pork, Chicken |
| Seafood | Fish, Shrimp |
| Dairy | Milk |
This multi-matrix capability addresses a long-standing challenge in residue monitoring: different sample types traditionally required different extraction protocols or entirely separate test kits. The new ELISA platform standardizes the process, saving laboratories time, money, and operational complexity.
Boldenone is a synthetic anabolic steroid sometimes illegally used in:
Livestock (beef, pork, chicken) – To promote muscle growth and improve feed efficiency
Aquaculture (fish, shrimp) – To accelerate growth in farmed seafood
Dairy cattle – Despite being banned in milk-producing animals
Human and animal athletes – Detected via urine, plasma, or serum samples
The ability to test all nine matrices with one kit gives regulatory bodies a unified workflow for everything from farm inspections to border control to sports doping enforcement.
Extensive validation studies demonstrate consistent, reliable performance regardless of sample type:
| Matrix | Detection Limit (LOD) | Recovery Rate (%) | Assay Time |
|---|---|---|---|
| Urine | 0.25 ppb | 85–110% | 90 min |
| Plasma/Serum | 0.15 ppb | 80–105% | 90 min |
| Beef | 0.025 ppb | 75–95% | 100 min |
| Pork | 0.025 ppb | 78–98% | 100 min |
| Chicken | 0.030 ppb | 76–96% | 100 min |
| Fish | 0.020 ppb | 80–100% | 100 min |
| Shrimp | 0.025 ppb | 72–92% | 100 min |
| Milk | 0.050 ppb | 70–90% | 90 min |
“Having a single ELISA kit that can handle urine, plasma, beef, shrimp, and milk is a game-changer,” said a laboratory manager from a European reference lab. “We no longer need to stock four different test kits or retrain staff for each matrix.”
The competitive ELISA principle remains consistent across all samples, with minor modifications to the extraction step:
Step 1: Sample Extraction (10–40 minutes)
Liquid samples (urine, plasma, serum, milk): Dilute and centrifuge. No organic extraction required.
Solid samples (meat, fish, shrimp): Homogenize, extract with organic solvent, evaporate, and reconstitute.
Step 2: Assay Procedure (60 minutes)
Add sample, enzyme conjugate, and antibody to pre-coated wells.
Incubate at room temperature for 30–60 minutes.
Wash to remove unbound materials.
Step 3: Signal Detection (20 minutes)
Add substrate solution. Color develops proportionally to bound enzyme.
Add stop solution.
Read absorbance at 450 nm (reference 630 nm).
Step 4: Result Interpretation
Intensity of color is inversely proportional to Boldenone concentration.
Compare against standard curve for quantitative results.
The kit's antibody shows excellent specificity while maintaining useful cross-reactivity for related compounds:
| Compound | Cross-Reactivity (%) |
|---|---|
| Boldenone | 100% |
| Boldenone Undecylenate | 85% |
| Testosterone | 12% |
| 1,4-Androstadiene-dione | 8% |
| Nandrolone | 5% |
| Methylboldenone | 4% |
| Trenbolone | < 0.1% |
| Cortisol | < 0.01% |
Note: The moderate cross-reactivity with testosterone and nandrolone makes this kit useful for broad-spectrum anabolic steroid screening, but positive results require confirmatory LC-MS/MS.
A national food safety authority launched a six-month surveillance program targeting anabolic steroid residues. The Boldenone ELISA Test Kit was used to screen 850 samples across all nine matrices.
| Matrix | Samples Tested | Presumptive Positives | Confirmed (LC-MS/MS) |
Pub Time : 2026-06-10 15:03:42
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Contact Details
Maoming Chenhui Biotechnology Co., Ltd.
Contact Person: Mr. Tina Tel: +8615818799493 |
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